Fig. 1

Notch1 expression is raised in 5-FU-resistant tissues of CRC patients and CRC cell lines. A The viability of SW620, SW620-R, HCT8 and HCT8-R cells was detected CCK-8 assay following 5-FU treatment for 48 h. B Apoptosis rates of SW620, SW620-R, HCT8 and HCT8-R cells were examined using flow cytometry analysis. C Western blot was used to detect protein expression of Notch1 in SW620, SW620-R, HCT8 and HCT8-R cells. D Immunohistochemistry staining was used to detect protein expression of Notch1 in normal, sensitive tumor tissues and resistant tumor tissues from CRC patients. E Immunofluorescence was used to detect protein expression of Notch1 in SW620-R and HCT8-R cells. ^*P < 0.05. ^**P < 0.01. ^***P < 0.001