Fig. 2

Silencing of Notch1 overcomes 5-FU-induced resistance in CRC cells. A Relative expression of Notch1 in SW620-R and HCT8-R cells was detected by qRT-PCR after transfection with si-NC or si-Notch1. B The proliferation of SW620-R and HCT8-R cells was assessed by EDU assay. C Apoptosis rates of SW620-R and HCT8-R cells were examined using flow cytometry analysis. D Western blot was used to detect protein expression of Bax, cleaved caspase-3, Bcl-2 and p53 in SW620-R and HCT8-R cells. E Relative expression of Notch1 in SW620 and HCT8 cells was detected by qRT-PCR after transfection with pcDNA-NC or pcDNA-Notch1. F The viability of SW620 and HCT8 cells was detected CCK-8 assay. G Apoptosis rates of SW620 and HCT8 were examined using flow cytometry analysis. H Western blot was used to detect protein expression of Bax, cleaved caspase-3, Bcl-2 and p53 in SW620 and HCT8 cells. ^*P < 0.05. ^**P < 0.01. ^***P < 0.001