Fig. 3

Silencing of Notch1 improves 5-FU sensitivity of CRC cells promotes pyroptosis by inhibiting glycolysis. A A Glucose Assay Kit from Nanjing Jiancheng was used to assess the level of glucose in culture medium of SW620, SW620-R, HCT8 and HCT8-R cells. B Western blot was used to detect protein expression of GLUT1, GLUT2, GLUT3 and GLUT4 in SW620, SW620-R, HCT8 and HCT8-R cells. C A Lactate Assay Kit from Nanjing Jiancheng was applied to assess the level of lactate in culture medium of SW620, SW620-R, HCT8 and HCT8-R cells. D The viability of SW620, SW620-R, HCT8 and HCT8-R cells was detected CCK-8 assay after glucose treatment for 24 h. E The viability of SW620, SW620-R, HCT8 and HCT8-R cells was detected CCK-8 assay after lactate treatment for 24 h. F A Glucose Assay Kit from Nanjing Jiancheng was used to assess the level of glucose in culture medium of SW620-R and HCT8-R cells. G A Lactate Assay Kit from Nanjing Jiancheng was applied to assess the level of lactate in culture medium of SW620-R and HCT8-R cells. H Western blot was used to detect protein expression of GLUT1, LDHA, PKM2, PGK1, HK2, ALDOA in SW620-R and HCT8-R cells. I Western blot was used to detect protein expression of NLRP3, Caspase-1, GSDMD-N, IL-18 and IL-1β in SW620-R and HCT8-R cells. ^*P < 0.05. ^**P < 0.01. ^***P < 0.001