Fig. 4

Notch1 influences 5-FU resistance by PCAF/HIF-1α-mediated glycolysis. A Western blot was used to detect protein expression of PCAF and HIF-1a in SW620, SW620-R, HCT8 and HCT8-R cells. B Immunohistochemistry staining was used to detect protein expression of PCAF and HIF-1α in sensitive tumor tissues and resistant tumor tissues from CRC patients. C Co-IP assay was used to examine direct interaction of PCAF and Notch1 in SW620-R cells. D Western blot was used to detect protein expression of PCAF and HIF-1a in SW620-R and HCT8-R cells. E Western blot was used to detect protein expression of GLUT3, LDHA, PKM2, PGK1, HK2, GLUT1 and ALDOA in SW620-R and HCT8-R cells. ^*P < 0.05. ^**P < 0.01. ^***P < 0.001