Fig. 9
From: Apoptosis-induced anticancer effect of transferrin-conjugated solid lipid nanoparticles of curcumin
Measurement of ROS. a Effect of dose on generation of ROS by CSSS, C-SLNs, Tf-C-SLNs, SS, and B-SLN in SH-SY5Y cells after 24-h treatment was determined by ROS assay using H2DCF-DA. Data as mean ± SD (n = 3). *p < 0.05, Tf-C-SLNs vs. C-SLN and CSSS; #p < 0.05, C-SLN vs. CSSS. b Effect of time of treatment on generation of ROS by CSSS, C-SLN, and Tf-C-SLN. SH-SY5Y cells were treated with all formulations (4 μM curcumin) and generation of ROS was determined at predetermined time points of 3, 6, 12, 24, and 48 h. Data as mean ±SD (n = 3). *p < 0.05, Tf-C-SLNs vs. C-SLN and CSSS; #p < 0.05, C-SLN vs. CSSS