Figure 2

CLSM images of cells incubated with dual-labelled nanoparticles containing Nile Red (red) (a, b, c) and p-HTAM (green) (d, e, f) after 8 min (a, d), 64 min (b, e) and 155 min (c, f) of incubation. Scale bars are 10 um. Nile Red was excited at 540 and fluorescence detected at 650–710 nm, while p-HTAM was excited at 405 nm and detected at 470–530 nm. The average mean Nile Red (●) or p-HTAM (▲) fluorescence intensities with standard deviation from cells (n= 42-46) is shown from every 10 min for the first half hour, and from approximately every half hour after addition of particles (g).