Figure 8
From: Contact-mediated intracellular delivery of hydrophobic drugs from polymeric nanoparticles
CLSM images of cells incubated with supernatant from a centrifuged nanoparticle suspension. Scale bars are 10 μm. Nile Red is shown in red. A fluorescence image obtained approximately 15 to 30 min after the addition of supernatant (a) and the corresponding transmission microscopy image (b) are shown together with a fluorescence image of untreated cells (c). Nile Red was excited at 561 nm, and fluorescence was detected at 575–700 nm.