Contact-mediated intracellular delivery of hydrophobic drugs from polymeric nanoparticles

Cancer Nanotechnology

Table 1 Rate constants (b) and maximum values (a) from regression curves f(t)= a(1 − e ^−bt ) fitted to data points from Nile Red uptake kinetics

Regression curve	Maximum value	Rate constant
Nanoparticles, normalised MFI	MFI 10.3	1.5 /h
Free Nile Red in medium, normalised MFI	MFI 2.7	-*
Free Nile Red in PBS, normalised MFI	MFI 2.5	-*
Nanoparticles, percentage positive cells	94%	4.9 /h
Free Nile Red in medium, percentage positive cells	37%	2.6 /h
Free Nile Red in PBS, positive cells	34%	2.4 /h

Curves were fitted to the percentage of positive cells and to MFI after incubation with free Nile Red in medium, free Nile Red in PBS, or nanoparticle-associated Nile Red.
*Regression curves fitted to average fluorescence intensity of cells incubated with Nile Red in cell medium or PBS had only two data points describing the initial increase and were therefore not reliable.

ISSN: 1868-6966