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Figure 1 | Cancer Nanotechnology

Figure 1

From: Erratum to: Contact-mediated intracellular delivery of hydrophobic drugs from polymeric nanoparticles

Figure 1

Representative CLSM fluorescence images and emission spectra of cells incubated with nanoparticles containing Nile red (a, b) or free Nile red (c, d) for 1 h. Scale bars are 10 μm. Nile red was excited at 488 nm, and fluorescence was detected from 520 to 700 nm. Examples of regions of interest where emission spectra were captured from the cytosol (squares) and vesicular structures (circles) are shown. From an emission-scan with an excitation wavelength of 488 nm, the emission spectra from the cellular areas of interest are shown together with the emission spectrum from Nile Red in nanoparticles as measured by spectrophotometry (b, d). In total, 8 cells incubated with nanoparticles were analysed with 12 regions of interest from the cytosol and 23 regions of interest from vesicles (b). Three cells incubated with Nile Red were analysed with 6 regions of interest from the cytosol and 8 regions of interest from vesicles (d).

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