Fig. 4
From: Cancer characterization and diagnosis with SERS-encoded particles
Adapted with permission from Lutz et al. (2008). Copyright 2008, American Chemical Association
a White light image of a formalin-fixed paraffin-embedded prostate tissue section stained with two COIN SEPs coded either with acridine orange (AOH) or basic fuchsin (BFU) and nucleic acid stain (YOYO). Each coin was functionalized with a different antibody anti-PSA (AOH-PSA) and anti-cytokeratin-18 (BFU-CK18). SERS mapping of b BFU-CK18 and c AOH-PSA. d Fluorescence mapping of YOYO. e Co-localization image that identifies epithelial nuclei (magenta) and co-expression of CK18 and PSA specifically in the epithelium (yellow).