Fig. 2

Effect of PVX-HER on the pathogenicity in N. benthamiana by SDS-PAGE, Western blotting, and RT-PCR. a SDS-PAGE patterns of proteins from N. benthamiana plants harvested at 3-week post-infection inoculation: Line 1: The single protein band (27 kDa) of purified PVX, Line2: Protein Marker US7 (GeneON, 310001), Line 3: The extract of N. benthamiana leaves was not inoculated, Line 4: the extract of leaves with symptoms which inoculated with free-PVX; showing 27 kDa band of PVX CP in different bands of plant extract protein. Line 5: the extract of leaves without symptoms which inoculated with PVX-HER; the evidence of different bands of plant protein without protein band-related CP of PVX. b Analysis of inoculation on N. benthamiana by Western blotting with PVX-specific antibody. Line 1: no band is shown from the extract of inoculated leaves with PVX-HER. Line 2: 27 kDa single protein band of PVX from the extract of inoculated leaves with free-PVX interacted with PVX-specific antibody, Line 3: no band is shown from the extract of non-inoculated N. benthamiana leaves as a negative control. c RT-PCR detection of the PVX CP gene in inoculated N. benthamiana. Line 1: the inoculated leaves of free-PVX are indicated the presence of 714 bp band-size. The position of the amplification products corresponding to the CP of PVX is also represented, Line 2: The inoculated leaves with PVX-HER showed no evidence of specific band (714 bp) PVX CP amplification, Line, 3: non-inoculated leaves (negative control), Line4: Thermo scientific MassRuler Low Range DNA Ladder (MAN0013019)