Algae-meditated route to cuprous oxide (Cu 2 O) nanoparticle: differential expression profile of MALAT1 and GAS5 LncRNAs and cytotoxic effect in human breast cancer

Table 1 Sequences and optimized concentration of primers used in this study

Primers	Primer Sequences (5′ to 3′)	Primer concentrations (nM)	Annealing temperature (°C)	Product size (bp)
GAPDH	AATGGGCAGCCGTTAGGAAA	300	55	168
GAPDH	GCGCCCAATACGACCAAATC	300	55	168
p27	TGGAGAAGCACTGCAGAGAC	300	53	252
p27	GCGTGTCCTCAGAGTTAGCC	600	53	252
p53	TACTCCCCTGCCCTCAACAAGA	300	55	181
p53	CGCTATCTGAGCAGCGCTCATG	300	55	181
bax	TCTGACGGCAACTTCAACTG	600	56	188
bax	TTGAGGAGTCTCACCCAACC	900	56	188
Bcl2	GGATGCCTTTGTGGAACTGT	300	63	236
Bcl2	AGCCTGCAGCTTTGTTTCAT	600	63	236
Caspase 3	CGGGGTACGGAGCTGGACTGT	300	58	251
Caspase 3	AATTCCGTTGCCACCTTCCTGTT	300	58	251
GAS5	TGAAGTCCTAAAGAGCAAGCC	900	52	120
GAS5	ACCAGGAGCAGAACCATTAAG	300	52	120
MALAT1	ATGCGAGTTGTTCTCCGTCT	300	52	118
MALAT1	TATCTGCGGTTTCCTCAAGC	300	52	118

ISSN: 1868-6966