Fig. 8

Protein and mRNA expression of p21 and p53 in MCF-7 cells after 24 h (top) or 48 h (bottom). The effect of SAHA and SAHA-loaded nanomicelles on p53 and p21 protein expression in MCF-7 cells was analyzed. Expression of all four markers was analyzed by western blot at 24 h (a) and 48 h (d). Data were normalized to the level of GAPDH. The effect of SAHA and SAHA-loaded nanomicelles on p21 and p53 mRNA expression was also done at 24 h (b) and 48 h (e), and normalized to the level of GAPDH, while the E- and N-cadherin mRNA analysis at 24 h and 48 h is shown in (c) and (f), respectively. The expression level is shown relative to the control as 1. A t test to test for statistical significance was performed (*p < 0.05, **p < 0.01, ***p < 0.001)