Graphene oxide nanofilm and chicken embryo extract decrease the invasiveness of HepG2 liver cancer cells

Cancer Nanotechnology

Table 2 Quantitative analysis of morphological images

Cell line	Group^a	Number of cells/mm²	SE	p-value	Area covered by cells (%)	SE	p-value
HS-5	Control	507	26.3	0.0139	36.4	1.42	< 0.0001
	nfGO	434			44.1**
	CELE	402^a			31.2^a
	nfGO + CELE	407^a			33.9
HepG2	Control	274	34.2	0.0112	32.7	2.98	0.0008
	nfGO	310			29.9
	CELE	246			41.8^a
	nfGO + CELE	396^a			48.9**
C3A	Control	245	33.0	0.0702	35.6	2.40	< 0.0001
	nfGO	306			23.9**
	CELE	351^a			54.1***
	nfGO + CELE	290			35.5

ImageJ software was used to calculate total cells area and number of cells
^anfGO: graphene oxide nanofilm, CELE: chicken embryo liver extract, significant *p < 0.05, **p < 0.01, ***p < 0.001

ISSN: 1868-6966