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Fig. 2 | Cancer Nanotechnology

Fig. 2

From: Curcumin-enriched Gemini surfactant nanoparticles exhibited tumoricidal effects on human 3D spheroid HT-29 cells in vitro

Fig. 2

Measuring the viability of HT-29 cells using MTT assay in a 2D culture system after 48 h (n = 8; a). Data showed that the viability of HT-29 cells was decreased by increasing the concentration of Gemini-Cur NPs. In this assay, the incubation of HT-29 cells with 60 µM and higher doses significantly decreases the viability of cells compared to the non-treated control cells. Evaluation of Gemini-Cur NPs on HT-29 cell spheroids after 48 h (b, c). Bright-field imaging showed the ability of HT-29 cells to generate spheroids by the hanging drop method. The incubation of spheroids with different doses of Gemini-Cur NPs contributed to the loss of spheroid integrity and structure. By increasing the dose of Gemini-Cur NPs, the diameter of spheroids was significantly increased and yielded a loose appearance (n = 5). We found statistically significant differences in the diameter of spheroids treated with 400 and 800 µM Gemini-Cur compared to the control cells. One-way ANOVA with Tukey post hoc analysis. **p < 0.01; ***p < 0.001

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