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Fig. 3 | Cancer Nanotechnology

Fig. 3

From: Curcumin-enriched Gemini surfactant nanoparticles exhibited tumoricidal effects on human 3D spheroid HT-29 cells in vitro

Fig. 3

Measuring the 3D spheroid HT-29 cells viability by LDH release after 48 h (a; n = 3). The incubation of HT-29 cell spheroids with Gemini-Cur NPs increased the release of LDH to the supernatant, indicating the loss of membrane integrity after being exposed to the Gemini-Cur NPs in vitro (n = 3). The uptake of Gemini-Cur NPs by 3D spheroid HT-29 cells was evaluated using flow cytometry analysis (b; n = 3). By increasing the Gemini-Cur NPs from 80 to 300 µM, the intracellular fluorescence was increased, showing the close relationship between Gemini-Cur NP concentration and fluorescence intensity. One-way ANOVA with Tukey post hoc analysis. *p < 0.05; *** p < 0.01

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