Fig. 6

The effect of Nano-PTC209 on the production of reactive oxygen species (ROS) in glioblastoma stem-like cells (GSCs) and U87MG (U87). Nano-PTC209-mediated ROS generation in GSCs and U87 cells was determined using H2DCFDA, which is a fluorescent reagent to detect ROS in cells. ROS induction in the GSCs and U87 cells following Nano-PTC209 treatment was detected by measuring fluorescent intensities in a microplate reader at 4, 8, and 24 h. Data are presented as the mean ± SD. * and *** indicate P < 0.05 and P < 0.001, respectively