Fig. 5

Co-administration of SPIONs with 5-FU increases macrophage infiltration and inhibits blood vessel formation in tumors. a Representative images of B16F10-tdTomato melanoma tumors treated topically with either vehicle (Saline), 5-FU alone, SPIONs alone or both 5-FU and SPIONs. Sections were stained for macrophages with anti-F4/80 antibody (brown). b Quantification of tumor-infiltrating macrophages from F4/80 immunostained sections. c Representative images of melanoma tumors treated topically with vehicle (Saline) 5-FU, SPIONs or both 5-FU and SPIONs. Sections were stained for endothelial cells with anti-CD31 antibody. The data are shown as the mean ± SEM of n = 5 measurements from 2 mice per group. d Quantification of vascularization from CD31-immunostained sections. One-way ANOVA followed by Sidak’s multiple comparisons adjustment indicating significant difference at *p < 0.05, **p < 0.01 and ***p < 0.001