Fig. 6

MAGCIT–MB internalization in cancer cells after treatment with nanocomplex (0.78 µg mL⁻¹ of MB, 6 h). a Analysis of the uptake of MAGCIT–MB by cells a A2780, b T-47D, c MDA-MB-231, d HUVEC and e HNTMC pre-treated with different inhibitors of specific endocytosis pathways; cytochalasin D (pinocytosis), nystatin (caveolin) and phenylarsine oxide (clathrin), cells without exposure to inhibitors and incubated with MAGCIT–MB (6 h) were used as a positive control. Data are presented as mean ± SD from three experiments. **p < 0.01, ***p < 0.001.* treatment compared to NT control. f Cancer cells ultrastructural images, obtained by Transmission Electron Microscopy (TEM), demonstrate the localization of MAGCIT–MB in lysosome/endosomes (black arrow) in cancer cell lines, in addition to the presence of cytoplasmic prolongations indicating phagocytosis (white arrow). Black arrowhead indicates the invagination of membrane, N nucleus, No nucleolus, Er Endoplasmic reticulum and R ribosomes. Scale bars = 5 µm and 1 µm