Fig. 5

Establishment of an in vitro blood–brain barrier (BBB) model. A Schematic of the in vitro BBB model; B morphological characteristics of the BBB model stained with sulforhodamine B (SRB). Representative image of an intact cell monolayer was captured under a Nikon light microscope (magnification, 100 ×); C transendothelial electrical resistance (TEER) values were evaluated 6 days after seeding; D permeability of fluorescein isothiocyanate (FITC)-dextran (MW 70,000) across the BBB model on day 5. Values are the mean ± S.D. (n = 3). *p < 0.05: compared with blank via Student’s t-test