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Fig. 4 | Cancer Nanotechnology

Fig. 4

From: Nanoparticles derived from insect exoskeleton modulates NLRP3 inflammasome complex activation in cervical cancer cell line model

Fig. 4

Relative expression of IL-1β gene and protein expression in HeLa 229 cells post-stimulation with LPS and ATP. The effect of three test doses (0.6 µg/ml, 6 µg/ml and 12 µg/ml) of CSNP and gCSNP, administered at the beginning and harvested at 4.5 h on A the expression of IL1β gene, relative to their expression in HeLa 229 cells stimulated with LPS (0 h) and ATP (3.5 h). B The expression of IL1β in supernatant/spent media of stimulated HeLa 229 cells. *p < 0.05, **p < 0.01 ***p < 0.001 and ****p < 0.0001. n ≥ 3. Significant upregulation was observed in the relative mRNA expression of IL1β gene (A) for 6 µg/ml (p < 0.0001, ~ twofold) and 12 µg/ml (p < 0.0001, ~ 12-fold) of gCSNP in the stimulated HeLa 229 cells. Interestingly, IL1β protein secretion from stimulated HeLa 229 cells (B) was observed to be significantly reduced in the presence of CSNP at 12 µg/ml (p < 0.0001) along with 6 µg/ml and 0.6 µg/ml (p < 0.01, respectively). However, we observed that the conjugation of gallic acid reversed the immunosuppressor mimicking action of CSNP, as all three gCSNP doses significantly (p < 0.0001) increased IL1β protein secretion in the extracellular solution of treated HeLa 229 cells

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