Fig. 2

Computed pH-dependences of the electrostatic component of the Gibbs free energy ΔG(pH) (left ordinate, curve 1, the two open circles indicate ΔG = 0) and the net charge nz(pH) (right ordinate, curve 2, the two open circles indicate the used in the experiments pH 6.5 and the isoelectric point nz = 0 at pH 10.6) of free equine cytC globule in aqueous medium. The two parts of the curve nz(pH) are colored according to the net charge sign: positive (blue) and negative (red)