Table 4 IC50 values of N. sativa essential oil (F0) and its nanoemulsions (F1 and F2) against HepG2 and Huh-7 hepatocellular carcinoma cells at different incubation time
Incubation time | IC50 and fold change | HepG2 | Huh-7 | ||||||
|---|---|---|---|---|---|---|---|---|---|
RDa | F0 | F1 | F2 | RD | F0 | F1 | F2 | ||
24 h | IC50 (µg/ml) | 100.0 | 98.39 | 65.27 | 55.74 | 100.0 | 90.44 | 52.38 | 35.5 |
Fold change (a) | – | 1.01 | 1.53 | 1.79 | – | 1.10 | 1.90 | 2.81 | |
Fold change (b) | – | 1.00 | 1.5 | 1.76 | – | 1.00 | 1.72 | 2.54 | |
48 h | IC50 (µg/ml) | 97.65 | 73.97 | 60.96 | 54.34 | 95.94 | 76.00 | 51.78 | 35.33 |
Fold change (a) | – | 1.00 | 1.21 | 1.36 | – | 1.00 | 1.47 | 2.15 | |
Fold change (b) | – | 1.32 | 1.60 | 1.80 | – | 1.26 | 1.85 | 2.72 | |
72 h | IC50 (µg/ml) | 92.61 | 66.23 | 49.26 | 45.10 | 89.21 | 45.10 | 41.14 | 25.00 |
Fold change (a) | – | 1.00 | 1.34 | 1.47 | – | 1.47 | 1.57 | 2.59 | |
Fold change (b) | – | 1.40 | 1.88 | 2.05 | – | 1.38 | 2.17 | 3.57 | |