Fig. 8

Cytotoxic effect of folate-conjugated polymers with or without presence of 5-FU against CRC cells. Viability of DLD-1, CaCo-2 and HT-29 cells after addition of bare folate-conjugated polymers (A, D, and G), folate-conjugated polymers with 5-FU (B, E, and H) and 5-FU (C, F, and I). Statistical significance for the folate-conjugated polymers without or with 5-FU or 5-FU vs. control was marked with (*); comparison of 5-FU concentration at free vs encapsulated form 5-FU 5 µg/mL vs 0.1 mg/mL + 5-FU 5 µg/mL marked with (#) and 5-FU 25 µg/mL vs 0.5 mg/mL + 5-FU 25 µg/mL marked with (^), p ≤ 0.05. The data presented constitute average results from three measurements ± SD