Fig. 4

As/miR34aNPs induced sublethal HCC pyroptosis. A Representative microscopic images of H-Huh7 and H-PLC cells treated with PBS, AsNPs and miR34aNPs in a normal physiological environment and As/miR34aNPs in normal and acidic environments. White arrowheads indicate the characteristic bubbles in the cell membrane (×400). B, C LDH release was assessed to measure cell membrane destruction. D, E Cell viability was detected by the MTT method. F, G The expression levels of caspase-3, GSDME, c-met and β-actin were assessed by Western blot analysis. *p < 0.05, **p < 0.01 compared with the control