Fig. 6

In vivo antitumour performance of different formulations in H-Huh7 tumour-bearing nude mice. A Tumour growth curves of different groups after treatment with the four formulations (control, AsNPs, miR34aNPs and As/miR34aNPs). Data are shown as the mean ± S.D. (n = 6). *p < 0.05, **p < 0.01 compared with the control. B Intratumoural fluorescence of Cy3-labelled miRNA (red) visualized by CLSM 24 h post-injection. The nucleus is stained with DAPI (blue). C, D Expression levels of GSDME, c-met, and Cyt-c in vivo were assessed by Western blot analysis. E Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and blood urea nitrogen (BUN) were assessed to evaluate the side effects of the different formulations after in vivo treatment