Fig. 6From: Non-homogeneous dispersion of graphene in polyacrylonitrile substrates induces a migrastatic response and epithelial-like differentiation in MCF7 breast cancer cellsAnalysis of MCF7 breast cancer cells migration by wound-healing assay using 35 mm Petri dishes with a 2 well insert. A–D Time-lapse microscopy images of wound closure of MCF7 cells growing on culture dishes (A, B, Control) or PAN/G10 membranes (C, D) at 0 and 24 h after culture insert removal. Note in panel B that migrating MCF7 control cells reduce the physical gap of the original wound (white lines) up to approximately 50% at 24 h. In contrast, panel D illustrates that the physical gap of the wound was unchanged after 24 h of insert removal when MCF7 cells were grown on PAN/G10 membranes. E Quantitative measurement of wound width confirmed no significant (n.s) changes in this parameter when MCF7 cells were grown on PAN/G10 membranes (0 h vs 24 h). Data from three independent experiments. Bars represent mean ± SD. ***p < 0.0005. Scale bar: 75 µm (A–D)Back to article page