Fig. 1

Preparation and characterization of PBAE/pDNA complex. A Polymer 446, a type of poly (beta-amino esters) (PBAEs), could deliver plasmid DNA efficiently. Here, PBAE 446 was used to bind and deliver plasmids encoding CXCL9, CXCL10, and CXCL11. B PBAE 446 was 100% effective at binding plasmid DNA at 5, 10, and 15 w/w ratios of polymer to DNA, the ratio of 10 was selected for further study. C Nanoparticle hydrodynamic diameter. The PBAE/pDNA complex was formed at 10 w/w ratios of polymer to DNA. D Representative SEM (scanning electron microscope) images of PBAE/pDNA complex. Scale bar, 100 nm