Fig. 3
Chemokines released by transfected LLC cells promote the invasion of T cells in vitro. The expressions of CXCR3 on CD4 + T cells (A) and CD8 + T cells (B) were examined by flow cytometry. T cells were isolated from the spleen and stained with APC labeled anti-CXCR3 antibody. CXCL9, CXCL10, CXCL11/CXCR3 axis mediate the invasion of T cells. C Transwell system was used to measure the invasion of T cells. 1 × 105 NPpCXCL9/10/11-transfected LLC cells and CFSE-labeled T cells were cultured in the lower and upper compartments, respectively. D Number of T cells in the lower compartment was examined 48 h later. Data are presents as mean ± S.D. *p < 0.05, **p < 0.01, and ***p < 0.001