Fig. 4

Infiltrating immune cell proportion alternation in pleural tumour after LMPs and SMPs treatment. A–C Methotrexate-loaded LMPs and SMPs in the treatment of LLC subcutaneous tumour model, immune cell infiltration in the tumour (A), the proportion of T cells in immune cells (B), the expression of granzyme B (C) and IFN- γ (D) in killer T cells. E–G Activation of dendritic cells by LMPs and SMPs. PKH26-stained LMPs and SMPs were incubated with bone marrow-derived DCs (BALB/c background), the average fluorescence intensity were detected by flow cytometry at different times (E), multiple of expression of dendritic cell surface molecules CD80 and CD86 after co-incubation for 24 h (F), percentage of proliferating cells after incubation of CFSE-stained dendritic cells with MPs (G). Student's t-test was used for data statistics, n = 3 in each group. The value is the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.005