Table 1 Primer sequences for real time qRT-PCR
Gene | Primer sequence | |
|---|---|---|
DR5 | F | 5’-CCAGCAAATGAAGGTGATCC-3’ |
R | 5’-GCACCAAGTCTGCAAAGTCA-3’ | |
FADD | F | 5’- CTCAGGTCCTGCCAGATGAAC-3’ |
R | 5’-GGACGCTTCGGAGGTAGATG-3’ | |
Caspase8 | F | 5’- AGAGTCTGTGCCCAAATCAAC-3’ |
R | 5’- CTGCTTCTCTCTTTGCTGAA-3’ | |
P53 | F | 5'-GTCTATAGGCCCACCCC-3' |
R | 5'-GCTCGACGCTAGGATCTGAC-3' | |
Bax | F | 5'-ATGGCTTCTATGAGGCTGAG-3' |
R | 5'-CGGCCCCAGTTGAAGTTG-3' | |
Bcl-2 | F | 5'-CTGCACCTGACGCCCTTCACC-3' |
R | 5'-CACATGACCCCACCGAACTCAAAGA-3' | |
NF-kβ | F | 5'-ATGGCTTCTATGAGGCTGAG-3' |
R | 5'-GTTGTTGTTGGTCTGGATGC-3' | |
STAT-3 | F | 5′-CATATGCGGCCAGCAAAGAA-3′ |
R | 5′-ATACCTGCTCTGAAGAAACT-3′ | |
GAPDH | F | 5'-GTCTCCTCTGACTTCAACAGCG-3' |
R | 5'-ACCACCCTGTTGCTGTAGCCAA-3' | |