Fig. 6From: Novel bispecific aptamer targeting PD-1 and nucleolin for cancer immunotherapyRecruitment of immunocytes to target cells with or without BiApt. Live nucleolin-positive MDA-MB-231 cells were stained green with CFSE, while activated PBMCs were stained red with eFluro670. Tumor cells and activated PBMCs were incubated together in the presence of BiApt (A), random DNA sequence (B), PD-1 aptamer (C), or AS1411 aptamer (D). The mixture was gently washed trice to remove unattached PBMCs, and the remaining cells were imaged by confocal microscopyBack to article page