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Fig. 3 | Cancer Nanotechnology

Fig. 3

From: Anti-EGFR conjugated nanoparticles to deliver Alpelisib as targeted therapy for head and neck cancer

Fig. 3

a and b Microscopy analysis of the interaction of NP and ACNP (20 µg/mL) with Cal33 cells. a Nuclei were stained with DAPI and pseudocolored in blue; cell membranes were stained with E-cadherin and pseudocolored in green; and DiR fluorescence signal was pseudocolored in red. Scale bar 10 µm. The images in the lower panel correspond to a sample not incubated with the primary anti-E-cadherin antibody. b The upper picture shows a magnification of the square outlined in white in a. The lower picture shows its corresponding 2.5D image. X and Y-axis indicate the position and Z-axis shows the fluorescence intensity value of each channel. c Flow cytometry histogram overlay plots showing the fluorescence of Cal33 cells treated with 1 (blue), 2 (red), and 20 (green) µg/mL NP or ACNP labeled with DiR for 6 h. The black line denotes unlabeled-cell background fluorescence. A representative experiment of three (with each condition in triplicate). d Number of positive cells in each condition. Mean ± SEM. pVal versus NP-DiR at similar concentration: **pVal < 0.001; *pVal < 0.05; ns, not significant

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