Fig. 4
From: Anti-EGFR conjugated nanoparticles to deliver Alpelisib as targeted therapy for head and neck cancer
Tumor targeting of DiR-labeled NP and ACNP in Cal33 xenografts. a Mouse xenograft model schematics. The indicated number of Cal33 cells was subcutaneously (s.c.) injected into the right flank of immunocompromised nude mice. After 3 weeks, mice were injected intravenously (i.v.) with 100 µl of DiR-labeled nanoparticles (NP-DiR and ACNP-DiR) or with non-labeled nanoparticles (ACNP) and ex vivo fluorescence was analyzed 48 h later. b Overlay of photographic and fluorescence images of Cal33 tumors from mice injected with the indicated nanoparticles (NP-DiR, ACNP-DiR, and non-labeled ACNP) and analyzed 48 h later. The color scale shows Fluorescence Intensity per mm2. Xtreme imaging system. c Net Fluorescence Intensity (sum of the background-subtracted pixel values within the region of interest -tumor- divided by its area). The signal from the tumor injected with non-labeled ACNP was subtracted from all sample (NP-DiR and ACNP-DiR) values. Mean ± SEM. **pVal < 0.001