Fig. 9

In vivo tumor reduction by radiotherapy. A Tumor volume plotted against time. B H&E staining of tumor tissue for the NC, RO, RL, RH groups after radiation. C TUNEL assay of the NC, RO, RL, RH groups after radiation. D Mean cell count of TUNEL-positive cells (green fluorescence) is represented as graph for the NC, RO, RL, RH groups after radiation. The data are presented as the mean ± standard deviation with number of samples (n) = 5 for tumor volume against time and n = 3 for mean cell count of TUNEL-positive cells. The mean tumor volume showed significant differences between the RO and Lipo-MGN-treated group (RL and RH) at day 21, as analyzed by the Student’s unpaired t-test. The mean cell count of TUNEL-positive cells showed significant difference between the RH and RL, as analyzed by the Student’s unpaired t-test. A two-tailed probability value (p) less than 0.05 was considered statistically significant. *Indicates p < 0.05, ** indicates p < 0.0001