Fig. 4
NPDOX/siPD-L1 existed anti-tumor effects on an H22 tumor-bearing animal model. a, b C57BL/6 mice were subcutaneously injected with H22 cells. After 1 week, tumor-bearing mice (n = 8) were intravenously injected with PBS, NPDOX, NPsiPD-L1 or NPDOX/siPD-L1, respectively. Tumor volumes were measured every 3 days (n = 8). At the end of the experiment, c, d the mRNA and protein levels of PD-L1 in tumor tissues were determined using RT-qPCR and western blot, respectively. e Histology examination of tumor tissue was performed using H&E staining. Besides, PCNA analyses were performed to determine the cell proliferation in tumor tissues. Scale bar was 10 μm