Table 3 Primers used to evaluate the expression of genes involved in proliferation and cell adhesion
Gene | Sequence of primer 5′→3′ | Amplicon size (bp) | GenBank accession number | References |
|---|---|---|---|---|
pcna | F: CCATCCTCAAGAAGGTGTTGG R: GTGTCCCATATCCGCAATTTTAT | 710 | NM_182649.2 | PrimerBlast |
ki67 | F: CCACACTGTGTCGTCGTTTG R: CCGTGCGCTTATCCATTCA | 123 | NM_001145966.2 | Szczepaniak et al. (2018) |
mcm2 | F: GTGGATAAGGCTCGTCAGAT R: GTCGTGGCTGAACTTGTT | 87 | NM_004526.4 | Szczepaniak et al. (2018) |
α1 | F: GGTTCCTACTTTGGCAGTATT R: AACCTTGTCTGATTGAGAGCA | 149 | NM_181501.2 | Dingemans et al. (2010) |
α2 | F: GGAACGGGACTTTCGCAT R: GGTACTTCGGCTTTCTCATCA | 154 | NM_002203.4 | Dingemans et al. (2010) |
α3 | F: AAGGGACCTTCAGGTGCA R: TGTAGCCGGTGATTTACCAT | 129 | NM_002204.4 | Dingemans et al. (2010) |
α4 | F: GCTTCTCAGATCTGCTCGTG R: GTCACTTCCAACGAGGTTTG | 131 | NM_000885.6 | Dingemans et al. (2010) |
α5 | F: TGCAGTGTGAGGCTGTGTACA R: GTGGCCACCTGACGCTCT | 88 | NM_002205.5 | Dingemans et al. (2010) |
α6 | F: TTGAATATACTGCTAACCCCG R: TCGAAACTGAACTCTTGAGGATAG | 113 | NM_000210.4 | Dingemans et al. (2010) |
αV | F: AATCTTCCAATTGAGGATATCAC R: AAAACAGCCAGTAGCAACAAT | 140 | NM_001145000.3 | Dingemans et al. (2010) |
β1 | F: GAAGGGTTGCCCTCCAGA R: GCTTGAGCTTCTCTGCTGTT | 107 | NM_002211.4 | Dingemans et al. (2010) |
β4 | F: AGACGAGATGTTCAGGGACC R: GGTCTCCTCTGTGATTTGGAA | 115 | NM_001321123.2 | Dingemans et al. (2010) |
Fak | F: CCCACCAGAGGAGTATGTCC R: CCCAGGTCAGAGTTCAATAG | 150 | XM_017013688.2 | Chen et al. (2012) |
e-cadherin | F: ACAACGCCCCCATACCAGA R: CACTCGCCCCGTGTGTTAGT | 138 | NM_001317185.2 | PrimerBlast |
n-cadherin | F: ACAGATGTGGACAGGATTGTGGGT R: TATCCCGGCGTTTCATCCATACCA | 124 | NM_001308176.2 | PrimerBlast |
β-catenin | F: CCTATGCAGGGGTGGTCAAC R: CGACCTGGAAAACGCCATCA | 95 | NM_001012329.2 | PrimerBlast |
gapdh | F: GAGAAGGCTGGGGCTCATTTG R: CATGGTTCACACCCATG | 97 | NM_002046 | PrimerBlast |