Table 1 Summary of antibodies used in immunocytochemistry and western blotting
Primary antibody | Cell target | Dilution | Clonality | Host species | Company | Secondary antibody |
|---|---|---|---|---|---|---|
Ki67 | Proliferation marker | 1:100 | Polyclonal | Rabbit | Abcam | Goat |
Nestin | Neural stem cells | 1:50 | Monoclonal | Mouse | Santa Cruz | Goat |
GFAP | Glial Tumor cells | 1:300 | Monoclonal | Mouse | Sigma | Goat |
CD34 | Endothelial cells | 1:20 | Monoclonal | Human | eBioscience | Mouse |
CD44 | Cancer progenitor cells | 1:50 | Monoclonal | Human | eBioscience | Rat |
CD45 | Hematopoietic stem cells | 1:20 | Monoclonal | Mouse | eBioscience | Goat |
NF-kB-p65 | Mammalian cells | 1:1000 | Polyclonal | Rabbit | Abcam | Goat |
p-Akt | Mammalian cells | 1:1000 | Polyclonal | Rabbit | Cell signaling | Goat |
p-SAPK/JNK | Mammalian cells | 1:1000 | Polyclonal | Rabbit | Cell signaling | Goat |
β-Actin | Mammalian cells | 1:1000 | Monoclonal | Rabbit | Cell signaling | Goat |