Fig. 4

Co-treatment of 5-FU with SPIONs increases necrosis and tumor-infiltrating lymphocytes in melanoma tumors. a Representative images of hematoxylin and eosin-stained sections of B16F10-tdTomato melanoma tumors that had been treated topically with either vehicle (saline), 5-FU alone, SPIONs alone or both 5-FU and SPIONs. b The extent of necrosis in the tumors. c The number of infiltrating lymphocytes per 1.3 mm² field in the tumors. The data are shown as the mean ± standard deviation of n = 3 mice per group. One-way ANOVA with Sidak’s multiple comparisons adjustment indicating significant difference at *p < 0.05 and **p < 0.01