Fig. 7
Cancer cell lines ROS generation by DCFH-DA assay after MAGCIT–MB (0.78 µg mL−1 of MB) treatment. A2780 (a) T-47D (b) and MDA-MB-231 (c) ROS production after 6, 24 and 48 h of exposure to nanocomplex. Data are presented as mean ± SD from three experiments. **p < 0.01, ****p < 0.0001.*Treatment compared to respective NT control. A2780 (d) T-47D (e) and MDA-MB-231 (f) DCFH-DA visualized by fluorescence microscopy after 6 h incubated with MAGCIT–MB. DNA counterstained with DAPI (blue) and cytoplasmic ROS generation with DCDH-DA (green). Scale bar = 50 µm