Fig. 5
As/miR34aNPs triggered Cyt c release. A Representative microscopic images of HEK293T cells treated with As2O3 after transient transfection with GSN-GFP or GSC-GFP. B GSN-mCherry and GSC-mCherry were transiently transfected into HEK293T cells. Then, the mitochondria and mCherry were observed by confocal microscopy, MitoTracker was used to stain the mitochondria, and Hoechst 33342 was used to mark the nuclei. C H-Huh7 cells were treated for 48 h with PBS, AsNPs and miR34aNPs in a normal physiological environment and As/miR34aNPs in a normal and acidic environment. The expression levels of Cyt c and Cyt c in the culture media (s-Cyt c) were examined by Western blot analysis