Fig. 5

Intraperitoneal ovarian tumor localization of Cy5.5 fluorescently labeled siRNA after administration of 5A2SC8 carrier. BLAB/c mice were injected intraperitoneally with SKOV3-GFP. Two weeks after tumor cell injection, mice were peritoneally injected with phosphate buffered saline (PBS), free Cy 5.5-siRNA or Cy5.5-siRNA-5A2SC8 nanoparticle with the dose of siRNA 1 mg/kg. Twenty-four hours later, mice were euthanized, and organs (heart, lungs, liver, spleen, kidneys, tumor) were removed. Tissues were imaged ex vivo for a GFP signal to identify luciferase expressing tumor cells and b Cy5.5 fluorescence to visualize the biodistribution of siRNA-containing nanoparticles, respectively. Scale bar: 1 cm