Fig. 5
From: Hyaluronan self-agglomerating nanoparticles for non-small cell lung cancer targeting
In vitro cancer cell targeting studies of the SAHNs. A Fluorescence-activated cell sorting (FACS) was performed in A549 cells and H23 cells of the human non-small cell lung cancer cell lines. FITC-labeled SAHNs were prepared with 0.1% (w/v) HA. B Confocal microscopy images of A549 (CD44-negative) and H23 (CD44-positive) NSCLC cells incubated with FITC-labeled SAHNs. 20 μg/mL FITC-conjugated SAHNs were added to each H23 and A549 cells line (1 × 106 cells), which was then incubated at 37 °C for about 4 h (scale bar = 50 μm). Notes: Fluorescent images of the cell nucleus stained by Hoechst 33,342 (white A, B), fluorescent images of FITC-SAHNs with green fluorescence in cells (white C, D), and fluorescent images of the FITC-SAHNs fluorescence superimposed on the nucleus and green fluorescence in cells (white E, F). HA hyaluronan, SAHNs self-agglomerating hyaluronan nanoparticles