Fig. 3
Effects of Zn0.3Fe2.7O4 NCs photothermia on the growth of H. pylori. Left panel: growth of a NCTC 11637, b clinical strain 27054, c clinical strain L2, after different treatment. H. pylori amount was measured by both colony count (CFU/ml) and absorbance of OD600. NCs plus heating group demonstrated significant inhibition effect on H. pylori growth in all strains, *** p < 0.001, compared with control. In contrast, interventions such as NIR, NCs without NIR irradiation, water bath at 41 °C, showed no growth inhibition. Right panel: growth of d NCTC 11637, e clinical strain 27054, f clinical strain L2 after different heating time and concentration of NCs. In equal heating time, NCs in higher concentration illustrated better H. pylori inhibition effects. When the NCs concentrations were equivalent, more heating time equaled better effects. The group of 50 ug/mL NCs photothermia at 41 °C for 20 min exhibited the best efficiency (n = 3), ** p < 0.01