Fig. 1
From: Tyrosine kinase inhibitor-loaded biomimetic nanoparticles as a treatment for osteosarcoma
2D biomimetic NP uptake and cytotoxicity on murine and human-derived OS cell lines. Representative images of NPs (Lipo and Leuko rhodamine labeled) internalization in murine (577, F420, RF379) (a, d, g) and human PDX-derived OS cells (TCCC-OS94, TCCC-OS202) (j, m) after 24-h treatment were observed using a Keyence BZ-X800 All-in-One Fluorescence Microscope. Merged images of green (FITC-WGA staining), blue (Dapi, nuclei staining), and red (rhodamine-NP staining) channels are shown. Scale bar = 10 μm. Relative uptake quantification of Lipo and Leuko in mOS (b, e, h) and in PDX-derived OS (k, n) cell lines after 24-h treatment. Ten images were analyzed for each condition. **p < 0.01; ***p < 0.001; ****p < 0.0001. Cell cytotoxicity analysis (MTT assay) of mOS (c, f, i) and hOS (l, o) exposed for 72 h to increasing concentrations of Pon, empty NP (Lipo and Leuko), and NP-loaded Pon (Lipo Pon, Leuko Pon). All data are expressed as mean ± SD (n = 3). Lipo and Leuko NPs for mOS, h-Lipo and h-Leuko NPs for human PDX-derived OS cells