Fig. 2
From: Tyrosine kinase inhibitor-loaded biomimetic nanoparticles as a treatment for osteosarcoma
3D biomimetic NP uptake and cytotoxicity on murine and human-derived OS cell lines. Representative images of Lipo and Leuko rhodamine-labeled NPs internalization at 24 h in mouse OS (577, RF379) (a, d) and PDX-derived OS (TCCC-OS94, TCCC-OS202) (g, j) cells using confocal Nikon A1 imaging system. Maximum projection images of green (FITC-WGA staining), blue (Dapi, nuclei staining), and red (rhodamine-NP staining) channels are shown. All data are expressed as mean ± SD (n = 8 spheroids per condition). Scale bar = 100 μm. Relative quantification of Lipo and Leuko NPs uptake in mOS (b, e) and hOS cells (h, k) spheroids after 24-h treatment. Cell cytotoxicity analysis (3D Cell Titer Glo assay) of mOS (c, f) and hOS (i, l) exposed for 72 h to increasing concentrations of Pon, empty NP (Lipo and Leuko), and NP-loaded Pon (Lipo Pon and Leuko Pon). All data are expressed as mean ± SD (n = 3). Lipo and Leuko NPs for mOS, h-Lipo and h-Leuko for human PDX-derived OS cells **p < 0.01; ***p < 0.001; ****p < 0.0001