Fig. 6

Abscopal effect on distal HT29 tumors. A Schematic diagram of the treatment of HT29 tumor-bearing mice. B Intratumoral immune cell infiltration of distal HT29 tumors 72 h post-treatment. Primary HT29 tumors were treated with saline, CPT-11, SH NPs, and SH/HA NPs plus 6 Gy of γ-ray, and the distal tumors were excised and analyzed for the relative abundance of different tumor-infiltrating immune cells by flow cytometry. Cells were gated appropriately to distinguish CD19⁺ B cells, CD11c⁺ DC cells, F4/80⁺ macrophages, DX5⁺/CD3⁻ NK cells, and DX5⁺/CD3⁺ NKT cells (n = 5). C Relative distal tumor volume and D tumor weight treated with saline, free CPT-11, SH NPs, and SH/HA NPs plus γ-ray irradiation (6 Gy) at 28 days (n = 5). *p < 0.05. **p < 0.01