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Table 4 Mean percentages of apoptotic, necrotic cells, and cell death

From: Preparation and characterization of cetuximab-loaded egg serum albumin nanoparticles and their uses as a drug delivery system against Caco-2 colon cancer cells

 

Viable cells (%

(Gate 1)

Early apoptosis (%)

(Gate 4)

Late apoptosis (%)

(Gate 3)

Necrosis

(%)

(Gate 2)

Cell death (%) (apoptosis + necrosis)

Early + Late apoptosis (%)

Control

84.6 ± 1.8

0.8 ± 0.1

10.0 ± 1.2

4.6 ± 0.5

15.4 ± 1.8

10.8 ± 1.3

ESA

90.2 ± 0.0

0.1 ± 1.7*

6.10 ± 0.1

3.6 ± 0.1

9.80 ± 0.2

6.20 ± 1.8

CET

82.3 ± 3.1

0.0 ± 0.0*

15.3 ± 2.8*

2.3 ± 0.3*

17.6 ± 3.1

15.3 ± 2.8*

ESA-NPs

66.2 ± 0.3*

0.5 ± 0.0

29.1 ± 0.2**

4.1 ± 0.1

33.7 ± 0.3*

29.6 ± 0.2**

CET-ANPs

40.0 ± 0.5**

1.6 ± 0.0**

46.0 ± 0.7***

11.8 ± 0.3***

59.4 ± 0.5***

47.6 ± 0.1***

CET + ESA-NPs

35.5 ± 1.0**

0.3 ± 0.1***

48.1 ± 1.9***

16.1 ± 0.3***

64.5 ± 1.6***

48.4 ± 1.9***

  1. Data are represented as mean percentages ± SD of three independent experiments. *Significance vs. control at P < 0.05; **: Significance vs. control at P < 0.001; ***Significance vs. control at P < 0.0001