Preparation and characterization of the myricetin-loaded solid lipid nanoparticles decorated with folic acid-bound chitosan and evaluation of its antitumor and anti-angiogenic activities in vitro and in vivo in mice bearing tumor models

Table 1 List of primers for real-time PCR

Test	Gene	Forward 5ʹ–3ʹ	Reverse 5ʹ–3ʹ
In vitro cytotoxic effect	Bax	TTTGCTTCAGGGTTTCATCCA	CTCCATGTTACTGTCCAGTTCGT
	Cas3	CTGGACTGTGGCATTGAGAC	ACAAAGCGACTGGATGAACC
	Cas9	CCAGAGATTCGCAAACCAGAGG	GAGCACCGACATCACCAAATCC
	Bcl-2	CATGTGTGTGGAGAGCGTCAAC	CAGATAGGCACCCAGGGTGAT
	GAPDH	TGCTGGTGCTGAGTATGTCG	GCATGTCAGATCCACAACGG
CAM assay	VEFG-A	GACCTGTAAATGTTCCTGCAA	AGAAATCAGGCTCCAGAAACA
	VEGFR-2	TCACGCCTTACAGACACCCT	AGGGAGATGTTACGGAGAATG
	β-actin	AGACAGCTACGTTGGTGATGAA	TGCTCCTCAGGGGCTACTCT
In vivo antitumor activity	TNFα	TTCTGTCTACTGAACTTCGGGGTGATCGGTCC	GTATGAGATAGCAAATCGGCTGACGGTGTGGG
	IL-6	CAAATTCGGTACATCCTC	CTGGCTTGTTCCTCACTA
	SOD	AGCATGGGTTCCACGTCCA	CACATTGGCCACACCGTCCT
	HER2	GCAGCTTCATGTCTGTGCC	ACAGAGACTCAGACCCTGGC

ISSN: 1868-6966