Fig. 3

Enhanced ferroptosis induced by MNV@DHA. A H22 cells were incubated different formulations (10 μg/mL of DHA) for 24 h followed by examining with fluorescence microscope (upper panel) and flow cytometry (lower panel). B H22 cells were treated with different formulations (10 μg/mL of DHA) for 24 h and then the cells were lysed for GSH determination. C H22 cells were performed with different treatments (10 μg/mL of DHA) for 24 h and then the protein was extracted for western blot assay. D H22 cells were incubated with different concentrations of the indicated formulations for 48 h and then cells were performed with CCK8 assay. E H22 cells were pretreated with or without ferrostatin-1 (Fer-1, 0.13 μg/mL) for 30 min and then treated with different formulations (10 μg/mL of DHA) for 48 h followed by PI staining for flow cytometric analysis